SpectraMax Quant dsDNA Assay Kits. For WST-1–based assay, 10 μL ready-to-use WST-1 reagent was added to each well and incubated for 3 hours at 37°C, and absorbance at 450 nm was measured. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se Figure 1: Schematic of a typical workow of the semi solid cloning process. Setup Guide on the Molecular Devices FilterMax™ F5 Multi-Mode Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. , a long-time leader in bioanalytical systems for microplate analysis, today announced the launch of two new multi-mode detection platforms that expand its portfolio of microplate readers, enabling the company to meet all combinations of customer needs now and into the future. Supplier: Molecular Devices. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . microplate reader. FilterMax F5 Multi-mode microplate reader. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Time-resolved Fluorescence. The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. Synergy™ H1 Hybrid Multi-Mode Microplate Reader, Wavelength Range: 200 nm to 999 nm, BioTek Instruments (CAT#:. 9). Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. com A. 4 Pages. Page 2 FilterMax F5 and F3 Multi‐Mode Microplate Readers Unpacking and Setup Guide 7. After further centrifugation in the same fashion, the supernatant was subjected to subsequent enzymatic reactions. cruzi incubated for 1 h with sertraline at 2 μM. The other members of the E39 family (525i, 530i, and 540i) do not have limited slip. Trinean Dropsense 96 Polychromatic. Plate chamber design of the SpectraMax category 1 microplate readers. USD ($) USD - United States Dollar. Bio-Tek Microplate Reader Synergy HT $8,995. Select preinstalled protocol on the GloMax® Instrument and pre-warm instrument. 5 fmol/100 μl fluorescein in Fluorescence Intensity-Bottom Read Mode, 15 amol/100 μl Europium in Time-Resolved Fluorescence Mode, 4 mP at 10nM Fluorescein in. The results are expressed as cell viabilities (%) based on the control condition. 2 Pages. 3 Pages. 8 Pages. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. Figure 2: Images of colonies of commonly used cell types raised in semi-solid culture in CloneMedia. Time-resolved Fluorescence. ScanLater Western Blot Detection System. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. 1 at 600 nm as determined using a FilterMax F5 multi-mode microplate reader (Molecular Devices). Easily accessible six-position filter slides enable wavelength selection from 340 to 650nm. 00The microplate reader market was valued at US$ 534. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. The plate was incubated for 1 hr at room temperature with shaking. IMAP assays are based on binding of. Activation of TLR4 and TLR2 receptors. com 8. Instrument Configuration: Assay Technology Adapta® & Setup for GeneBLAzer® Assay on Molecular Devices FilterMax™ F5 Microplate Reader with SoftMax® Pro 6. Cells were incubated at room temperature in the dark for 10 min, then luminescence was detected and quantified with the FilterMax F5 Multi-Mode microplate reader (Molecular Devices, Sunnyvale, CA, USA). PerkinElmer Multimode Plate Reader EnSpire 2300 $8,495. Click “Eject Plate Carrier. Learn how to set up protocols, customize reports, export results, and more with this PDF document. 40266 Email: [email protected] or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers begining with. Molecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life Technologies’ GeneBLAzer® assays. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Fred A. The following document is. 00. 00. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Installation Qualification (IQ)Plates were read at absorbance 405 nm using a FilterMax F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. The amount of colorimetric product was continuously monitored for 120 min. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. A blank sample was prepared by mixing. State-of-the-art Multi-Mode Microplate Reader. Drug repurposing has been an interesting and cost-effective approach, especially for neglected diseases, such as Chagas disease. used. A) CHO colonies, serum-free suspension-adapted, in CloneMedia-CHO. Categories:. This instrument has passed specific verification criteria. Approximately 50 µl of these. Molecular Devices technical support. 2 μM ATP standard was. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. Match case Limit results 1 per page. . Measurements of Caspase-3/7 Activity. Absorbance was measured at 485 nm with FilterMax F5 Multi-Mode microplate readers and the percentage of viable cells was calculated in relation to cells cultured in pure medium. Transfluor Technology. Statistical analysis was performed using. MetaFluor Software. FilterMax F3 & F5 Microplate Readers. $3,000 USD. 3 Pages. FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate ReaderThe upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. 003 fmol 0. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The amount of colorimetric product was continuously monitored for 120 min. LabX is a multi-vendor marketplace that connects you with the life science tools and chemistry you need to run your lab. Installation Qualification (IQ) FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and. The cells were washed again, and 90 µL of a substrate solution was added to the wells for 15 min. Fold changes were calculated as the area under the curve of stimulated HKLs relative to unstimulated HKLs (RPMI without zymosan). The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. The haemolytic activity was determined in the cell supernatant by optical density readings at 570 nm (FilterMax F5 Multi-Mode Microplate Reader, Molecular Devices). The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Set up plate with cells, reagent and test compounds. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader w/ One Year Warranty (Svc Contract Available)Find used microplate readers on Machinio to perform a wide range of assays including ELISAs, cell viability assays, nucleic acid quantification, and more. Turn on the power switch on the back of the instrument. 030 fmol Fluorescence Polarization (FP) (Fluorescein 1 nM, SD) 96-well (200 µL) 0. ~ Bio-Tek ELx808 Microplate Reader $2,795. An MTT assay was performed with 5 mM BHB in the presence or absence of LPS (1 µg/mL) for 24 h. MIA PaCa-2 cells were cultured in 96-well plates with 1000 cells/well. Plates were read at absorbance 405 nm using a FilterMax F5 multi-mode microplate reader (Molecular Devices, Sunnyvale, CA). Cytokine concentration was determined by plotting the OD or median fluorescence intensity (MFI) of each sample on each cytokine concentration curve and multiplying the result by a dilution. com 8. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. They are very complex instruments. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. Molecular Devices Multi-Mode Microplate Reader FilterMax F5 $7,995. 4 Pages. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Unsuccessful migration. Multi-Mode Microplate Reader. We specialize in GC, IC, GC/MS, HPLC, LC/MS, Spectrophotometer, and PCR Systems. FilterMax® F3 & F5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell viability, ELISA, Fluorescence,. 00 USD. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader. Recommended Optics P/N Filter information 6590-0048 FilterMax™ Fluorescence Excitation Filter 405 nm (bandwidth 30 nm) FilterMax F3 Multi-Mode Microplate Reader Includes Excitation Filter Slide #3 (Absorbance: 405 nm, 450 nm, 492 nm, 620 nm; Fluorescence: 360 nm, 485 nm), Emission Filter Slide #3 (Fluorescence: 465 nm, 535 nm), Multimode Analysis Software, Power Cable, RS-232 Serial Cable, User’s Manuals FilterMax F5 Multi-Mode Microplate Reader Specialized microplate readers are available that enable HTRF (Homogeneous Time-Resolved Fluorescence) experiments and analyses. SpectraMax Paradigm Multi-Mode Safety Guide; SpectraMax Paradigm Multi-Mode Unpacking Guide; SpectraMax Paradigm Multi-Mode. ( C ) H2A. 4 Pages. It can be upgraded into a powerful multi-mode reader with up to seven detection modes. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Renilla luciferase activity was normalized to the background Firefly luciferase activity produced from the same psiCHECK-2 vector for each condition. Renilla/Firefly luciferase activities were measured twice. In addition, the instrument features linear and orbital shaking, plus temperature control. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Instrument Configuration: Assay Technology Adapta® & After 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. Activation of TLR4 and TLR2 receptors. The absorbance was read with a spectrophotometer (Filter Max F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA, USA) at a wavelength of 595 nm. BioStack 4 offers patented plate de-lidding and re-lidding for sensitive cell-based workflows. Recommended Optics The lysates were incubated in caspase-3 substrate Ac-DEVD-pNA at 37 °C. The absorbance of samples at 405 nm (A 405) was determined at room temperature at 20 and/or 60 min after addition of substrate using a FilterMax F5 Multi-Mode Microplate reader (Molecular Devices, San Jose, CA). Labsystems Fluoroskan Ascent FL Microplate Fluorometer $3,250. 561. 0. The drawing shows a standard microplate of 15 mm height covered with a lid. GFP expression differences. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. Neurotransmitter Transporter Uptake Assay Kit. FAST AND ACCURATE METERTECH Microplate Reader $3,650. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. 100 fmol 1536-well (8 µL) 0. performs within its specifications. Ammonia and NO 2 Ϫ / NO 3 Ϫ analyses were conducted according to established protocols (37, 38), using a FilterMax F5 multimode microplate reader (Molecular Devices, Canada). Each kit was used on the same day that its reagents were reconstituted. After 2 hours, each well was transferred to a 96-well flat-bottom plate and the absorbance at 490 nm was measured on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA). Caspase-3/7 activity, an indicator of apoptosis, was measured by a luminescence assay using caspase-Glo. The following multi-step binary elution gradient, based on an water as phase A and acetonitrile as phase B, both containing 0. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. A standard curve was created by diluting the ATP standards to a range between 2 × 10 −3 and 20 μM ATP. They provide absorbance, fluorescence, glow luminescence, and other read modes for. Each condition was performed in. Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. It allows the user to choose between monochromator-based optics for flexibility or filter-based optics for sensitivity. Plate Formats:. 3 Pages. 3. View specifications, prices, citations, reviews, and more. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers; SpectraMax QuickDrop Micro-Volume Spectrophotometer; EMax Plus Microplate. 3. Ease of use. BMG LABTECH. Besides the number of substrates used by microbiota,. Plaque reduction. The values of luminescence are directly proportional to the number of viable cells. A powerful and reliable microplate reader is what you need! Get access to readers which will ensure an optimal readout. Scan measurement types: endpoint, kinetic, multiple wavelength, linear scan and area scan. With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. F3/F5 Multi-Mode Microplate Readers The FilterMax Series of microplate readers includes: FilterMax F3 Multi-Mode Microplate Reader. The multi-mode microplate reader provides flexibility and ease of use for a variety of applications Market Highlights Microplate Readers Market is predicted to project a notable CAGR of 4. Sign In Upload. Biofilm quantification and scanning electron microscopy (SEM) analysis. They provide absorbance, fluorescence, glow. F3/F5 Multi-Mode Microplate Readers. In the list below the slide number 21, select the positions where the emission filters are installedF3/F5 Multi-Mode Microplate Readers. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . A powerful and reliable microplate reader is what you need! Get access to readers which will ensure an optimal readout. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumAfter 30 min, the absorbance of the lysates was measured at 405 nm using a FilterMax F5 Multi-Mode microplate reader. 25. F3/F5 Multi-Mode Microplate ReadersThe plate was incubated for 1 hr at room temperature with shaking. Fluorescence intensity was measured with Filter Max F5 Multi-Mode Microplate Readers (Molecular Devices) at an excitation wavelength of 535 nm and an emission wavelength of 590 nm. Protease activities were calculated from the slope of the linear portion of the normalized fluorescence vs. . S. ] Example of document to evaluate if the reader . Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of performing absorbance, luminescence, and fluorescence intensity measurements on microplate samples (Figure 1-1). 4 (Molecular Devices, Sunnyvale, CA, USA) at room temperature (fluorimeter settings used were as follows: measurement type, kinetic, 60 cycle, 60-s interval; excitation wavelength, 360 nm;. Visible absorbance -. The ucs load command creates a backup of. ABS Test results. Fluorescence kinetics were monitored from the top in a FilterMax F5 Multi-Mode Microplate Reader and the Multi-Mode Analysis Software 3. Cells were co-transfected with 2 μg total plasmid DNA (1760 ng empty vector or proviral plasmid DNA. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. Figure 1. 05); b Evaluation of the ATP levels of T. x phosphorylation of wild-type BY4741 yeast exposed to 1 μg/ml fludioxonil in 1% DMSO or 0. After 2–4 h incubation at 37 °C, the medium was removed and 100 μl dimethyl sulfoxide (DMSO) was added to each well to dissolve the formazan. The OD 600 was plotted against time using Graphpad Prism 8. 6 reviews. Absorbance values recorded at 490 nm using a microplate reader (FilterMax F5, Multi-Mode Microplate Reader, Molecular Devices) were then analyzed with Excel software (Office, Microsoft) and GraphPad Prism 6 software (La Jolla, CA, USA) to assess statistical significance. 9 mM H 2 O 2 for. The MTT assay was performed with 100 μM vitamin E in the presence or absence of LPS at a concentration of 1 µg/mL for 24 h. 3 Pages. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Instrument Configuration: Assay Technology Adapta® & The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. 713579). The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe OD 600 of the culture in the plate was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 20 min over 20 h. FilterMax® F3 & F5 Multi-Mode Microplate Readers. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 3. The instrument performs an initialization procedure that moves the optics and the microplate drawer to home positions. 3 Pages. The OD 600 of the culture in the plate was measured by a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) every 20 min over 20 h. Other technologies such as imaging. FilterMax™ F3 and FilterMax™ F5 Multi-Mode Microplate Reader User Guide 5008528 A September 2010 DTX Multimode Readers User Guide. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $ 7,900. To contact technical support by phone in the U. We have 2 Molecular Devices FilterMax F5 manuals available for free PDF download:. 2. Chemiluminescence emission was measured in real time (every 2 min for 120 min) with a FilterMax F5 Multi-Mode Microplate Reader at 27°C and expressed as area under the curve, as previously described . 3 Pages. The hemolysis was calculated with the following formula: Hemolysis (%) = (A sample − A PBS)/(A Triton − A PBS. Not for use in diagnostic procedures. All experiments were performed in triplicate. Caspase-3 activity was used as a marker of cell apoptosis and was assessed according to. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. MetaFluor Software. J. shopping cart 0 items $0. 4 Pages. In the case of cocultures, donor and recipient. shopping cart 0 items $0. BMG POLARstar Microplate Reader, 115-230V, BMG Labtech (CAT#: STEM-SPO-0061-YJL) Perkin Elmer Victor3 1420 Multilabel Counter, 110-240V, 3 Amps, PerkinElmer (CAT#: STEM-SPO. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 3 Pages. An extra plate was done with all the serial dispersions for the MgO-NPs (in. 3 Pages. MetaMorph NX. SpectraMax® M5/M5e Microplate Reader & FlexStation® 3 Benchtop Multi-Mode Microplate ReaderSpectraMax M-series Microplate readers. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 3 Pages. 4 Pages. DCF fluorescence was measured by using a microplate reader (FilterMax F5) at maximum excitation and with emission spectra of 485 nm and 535 nm, respectively. A photomultiplier tube (PMT) converts a light pulse into an electrical signal of measurable magnitude. A Filter Max F5 Multi-Mode Microplate Reader was used to measure absorbance at 595 nm. 5. Learn More. Cells were harvested for luciferase assay 48 h after transfection. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se The method is based on the reaction of malondialdehyde (MDA) with thiobarbituric acid (TBA) under conditions of high temperature and acidity that generates a fluorochrome, which is quantified by fluorimetry (F5 Multi-Mode Microplate Reader), with excitation at 515 nm and emission at 553 nm. MICROPLATE READERS Multi-Mode Readers. Page 137 Using the same plate as was used in step 3, pipette 50 µL/well of buffer • into wells C1–F5 and C10–F12. Click on the microplate icon in the upper left corner and then click FilterMax™ F5 Multi-Mode Microplate Reader: Supplier No. Products : General, FilterMax F5 reader. Turn on the power to the FilterMax F5. After 30 min, absorbance of the lysates at 405 nm was measured on a microplate reader (FilterMax F5 Multi-Mode microplate reader). Read Review (1)FlexStation® 3 Multi-Mode Microplate Reader. Supernatant (25 μL), or standard, was added and mixed. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-The EMax Plus measures flat and round 96-well microplates. 100 fmol 1536-well (8 µL) 0. , San Diego, CA, USA). 00. Protein quantification was carried out using the Pierce™ BCA Protein Assay Kit (Thermo Fisher) and a FilterMax F5 Multi-Mode Microplate Reader (San Jose, CA, United SA). time plots at 30, 60, and 90 min using MATLAB® R2017b (9. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:FilterMax F5 Multi-Mode Microplate Readers, 96 to 384 Well or 6 to 1536 Well, Molecular Devices, LLC (CAT#: STEM-LE-0509-LC) Mithras LB 940 Multimode Microplate Reader, 200-1000 nm Spectral Range Absorbance, BERTHOLD TECHNOLOGIES GmbH & Co. Page 1 Hybrid Multi-Mode Microplate Reader Synergy ™ Operator’s Manual. , Sunnyvale, CA, USA) and normalized to the. Cells were seeded in 24-well plates and cultured. Transfluor Technology. Fluorescence was measured using a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). The microplate drawer door must be held open manually while. & FlexStation® 3 Benchtop Multi-Mode Microplate Reader : Learn more about the SpectraMax® M5 and the SpectraMax® M5e;. SpectraMax Paradigm Multi-Mode Microplate Detection System from Molecular Devices, LLC. 4. The mixture was then boiled for 10 min. Learn more about the FilterMax™ F5 Multi-Mode Microplate Reader . Devices FilterMax Multi-Mode Microplate Readers. • DNA/RNA quantitation • Reporter gene assay The FilterMax™ F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), and time-resolved fluorescence (TRF) for 6- to 1536-well plates. Our SpectraTest® ABS2, FL1, LM1, and Multi-Mode Validation Packages provide automated,A delightfully easy to use multimode reader. After incubation, each microplate was analyzed for absorbance reading. SpectraMax QuickDrop UV-Vis Spectrophotometer. After the absorbance was measured at 450 nm on a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA), the concentrations of the samples were determined by extrapolation from a standard curve made in a four-parameter nonlinear regression program in Softmax Pro software (Molecular Devices). They provide absorbance, fluorescence, glow luminescence, and other read modes for Affordability and unmatched assay performance in filter-based microplate readers. CloneMatrix. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). searchPolymerization and polymerization kinetics were monitored in a Corning 96-well black flat bottom polystyrene microplate (Corning, Lowell, MA, USA) using a FilterMax F5 Multi-Mode Microplate Reader. Transfluor Technology. The quantity of colorimetric product was continuously monitored for 120 min. MetaMorph NX. 425 or. The amount of each HTRA1 protein was. com A. F3/F5 Multi-Mode Microplate ReadersCell pellets were lysed and HTLV-1 LTR-transactivation was measured via luciferase assay according to the manufacturer’s protocol (Dual-Luciferase ® Reporter Assay System, Promega Corporation, Madison, WI; Filtermax F5 Multi-Mode Microplate Reader, Molecular Devices, San Jose, CA) . MetaFluor Software. SpectraMax Glo Steady-Luc Reporter Assay Kit. A FilterMax F5 multi-mode microplate reader (Molecular Devices, San Jose, USA) was used to determine the absorbance (OD 450nm -OD 570nm). 50/50. Turn on the power switch on the back of the instrument. --> Life sciences. Products : General, FilterMax F5 reader Please click on the links below to view the Microplate Reader Comparison Tables. Selecting your dsDNA assay kits The family of SpectraMax Quant dsDNA Assay Kits consist of three kits each designed for a specific range of DNA concentrations and optimized for use with florescenceThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The next day, precultures were diluted to an optical density of 0. 6 Pages. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $7,900. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Multi-Mode Detection Platform Overview The FilterMax™ F3 and FilterMax™ F5 Multi-Mode Detection Platforms are computer-controlled multi-mode detectors capable of. FilterMax F5 Multi-Mode Microplate Reader. Images were captured using Molecular Devices CloneSelect Imager at a suitable time point post plating. SpectraMax Glo Steady-Luc Reporter Assay Kit. The amount of the colorimetric product was monitored continuously for 120 min. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. 9. $3,000 USD. Synergy HT laboratory equipment pdf manual download. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. Absorbance mode reads plates up to 0. , Sunnyvale, CA, USA). The data were analyzed using Multi-Mode Analysis software (Molecular Devices, Corp. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. 030 fmol Fluorescence Polarization (FP). 2 Pages. Microplate readers, multi-mode, FilterMax™ F3 and F5 Supplier: Molecular Devices Description: The FilterMax™ series of multi-mode microplate readers can be operated as standalone instruments or can be integrated seamlessly with laboratory autom. The following document is intended to demonstrate setup of this instrument. 100 fmol 384-well (75 µL) 0. How to Get Multi-Day Kinetic Data. Once the FilterMax F5 has completely powered on, double-click on the Multi- Mode Analysis Software. ; Page 2 For research use only. 4. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. . c. The FilterMax F5 features absorbance (UV and visible), fluorescence intensity - FI (top and bottom reading), glow luminescence, fluorescence polarization (FP), time-resolved fluorescence (TRF), for 6- to 384-well plates. Each measurement was repeated at least 3 times, and IC 50 values were calculated by non-linear regression in GraphPad Prism 9. FilterMax F5 Multi-Mode Microplate Reader. Luminescence intensity was measured with a FilterMax F5 Multi-Mode Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). For customers outside the U. 5 ml 0. 00 USD. Instrument Configuration: Assay Technology Adapta® & LanthaScreen® EuropiumThe parasite viability was determined by resazurin assay (0. . FilterMax F3. MS-3 Laser Bar Code Reader. VisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate Data Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. 012 fmol 0. --> Life sciences. The fluorescent signals were measured at 10 minutes and one hour after the Stoplight Red substrateFilterMax F5 Multi-Mode Microplate Reader (Molecular Devices) 12. Related Instruments Agilent BioTek BioStack microplate stacker Automate routine microplate washing or dispensing processes with the compact BioStack microplate stacker. Luminescence (glow) Fluorescence polarization. 7/4/2018 Great Multi-Mode Microplate Reader Chirag Patel Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. The readings of samples incubated with different carbon sources were subtracted from the average of the control readings. Transfluor Technology. F3/F5 Multi-Mode Microplate Readers. The increase in NADH, an end product in this reaction, was determined by.